QHight troughput quantification of alternatively spliced isoforms
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This project is conducted at Laboratoire de génomique fonctionnelle de 'Université de Sherbrooke (LGFUS). The overall goal of our project is to annotate and analyze the splice isoforms of cancer-related genes to understand in a more complete manner the mechanism of oncogenesis. This information will have tremendous value since novel markers will be identified for cancer diagnosis, and novel targets will become available for drug discovery programs. A rigorous assessment of the physiological impact of tumor-specific alterations in alternative splicing is crucial to understand the contribution of splice isoforms to specific types of cancer. To assess the functional importance of each isoform that belongs to cancer-related genes, strategies capable of decreasing the abundance of specific mRNA isoforms or specifically reprogramming splice site selection will be of tremendous value and may turn out to have important clinical relevance. For example, shifting the balance of isoforms in favor of variants displaying dominant negative activity may help reverse the malignant phenotype of cancer cells. |
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